Protein a can be applied to the anion exchange chromatography matrix by methods well known in the art.
通过本领域公知的方法可以将蛋白a施加到阴离子交换色谱基质上。
The solid sialic acid was obtained by lyophilization after counteraction, filtration and separation using ion exchange chromatography.
水解液中和过滤后，用离子交换色谱分离与冷冻干燥得到唾液酸产品。
Selenium-containing phycocyanin(Se-PC)was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography.
使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。
In this review, the application of anion exchange chromatography to pharmaceutical plasmid analysis was reviewed.
本文对阴离子交换色谱在药用质粒分析方面的应用与研究进展进行综述。
High purity ovotransferrin was extracted from albumen by precipitation with ammonium sulfate and ion exchange chromatography in the study.
采用硫酸铵沉淀法和阴离子交换层析法从鸡蛋清内提取高纯度卵铁传递蛋白。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
The method of high performance anion exchange chromatography with pulsed electrochemical detection (HPAEC-PED) was reviewed.
评述了高效阴离子交换色谱和脉冲电化学检测方法。
The iron-binding capacity of lactoferrin was 87.7% produced by ultrafiltration and strong cation exchange chromatography. Its iron saturation point was 15.1%.
采用超滤-离子交换色谱法制备的乳铁蛋白的铁结合能力为87.7%，铁饱和度为15.1%。
A new method of ligand exchange thin-layer chromatography for the resolution of amino acid enantiomers has been studied.
报道了一种新的配体交换薄层色谱拆分氨基酸对映体的方法。
A silica gel-ion exchange chromatography has been developed for the analysis of polar components in the used gasoline engine oil.
本文建立了汽油发动机油在用油极性组分的分析方法，即硅胶-离子交换色谱法。
Based on this media, a new hydrophilic weak cation exchange stationary phase(WCX) for high(performance) liquid chromatography was synthesized by a new chemical modification method.
该微球经化学改性后得到一种亲水性良好的新型高效弱阳离子交换色谱固定相。
Single peak insulin was purified by using DEAE-Sepharose fast flow ion exchange chromatography and crystallizing with zinc component.
单峰胰岛素经deae琼脂糖快流速离子交换层析后用醋酸锌结晶，得到结晶单组分胰岛素。
The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated.
研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白i的最佳条件。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
The paper discussed the separation of Xylitol, Arabitol and Sorbitol by Ion Exchange Chromatography.
本文研兖了木糖醇、阿糖醇、山梨糖醇在离子交换树脂层析柱上的分离。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Pure enzyme was obtained by using the salt fractionation with ammonium sulphate, the ion-exchange chromatography and the gel filtration.
使用硫酸铵分级盐析，离交色谱，凝胶过滤可获得纯净的酶。
In addition, an original heterogeneous polymerization method, based on displacement chromatography, has been tested. We named it "polymerization reaction in cation exchange chromatography".
此外，建立了一种原创的基于置换色谱的非均相聚合方法——阳离子交换色谱柱上聚合反应。
Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.
建立了配体交换色谱手性流动相法拆分茶氨酸对映体的方法。
The development in the field of separating proteins using ion exchange chromatography was reviewed with 100 examples.
综述了离子交换色谱在蛋白质分离方面的新进展。参考文献100余篇。
A method for determination of iodide in seawater by high performance anion exchange chromatography with ultraviolet detector (HPAEC-UV) in basic system has been established.
建立了在碱性体系中采用高效阴离子交换色谱和紫外检测器联用测定海水中碘离子的分析方法。
Objective To explore the possibility of achieving the highest resolution by using combination of anion and cation exchange chromatography.
目的：探索以阴、阳离子交换色谱串接组合为方式，进行高分辨率分离纯化蛋白质的可行性。
PurposeTo explore the possibility of combination of anion and cation exchange chromatography by using the polarity organic solvents in eluent.
目的研究极性有机溶剂辅助阴、阳离子交换色谱串接组合纯化蛋白质的可行性。
Cation - anion exchange resin can crack and disassemble bond - chromatography, doff the bond - large - molecule and dissociate paclitaxel.
阴阳混合离子交换树脂可以使键合态紫杉醇发生裂解，脱去键合的大分子物质，游离出紫杉醇。
OBJECTIVE:Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography.
目的：选择多核苷酸磷酸化酶快速离子交换层析的最佳条件。
HbA 1c; low performance liquid chromatography (LPLC); ion exchange chromatography (IEC).
糖化血红蛋白；低压液相层析；离子交换层析。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The results showed that the methods of isoelectric precipitation, gel-filtration chromatography and anion-exchange chromatography were suitable for purifying the major allergens in buffalo milk.
实验结果表明，等电点沉淀、凝胶柱层析法和阴离子交换法适合于分离水牛乳中主要过敏原成分。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。
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