With availability of a fragment of target DNA sequence, flanking unknown DNA sequence was obtained rapidly by successive chromosome walk using Il-PCR method.
运用该技术进行染色体连续步移，快速扩增DNA侧翼未知序列。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
The result showed that this IPCR technique is quick, convenient and stable for flanking sequence cloning.
实验结果表明这个方法具有快速、稳定和高效的优点。
On the basis of inverse PCR, we have founded a IPCR technique to clone foreign gene's flanking sequence in transgenic rice, which is suitable to treat mass materials.
以反向pcr (IPCR)为基础建立了适合于处理大量材料的克隆转基因水稻中外源基因旁侧序列的技术体系。
On the basis of inverse PCR, we have founded a IPCR technique to clone foreign gene's flanking sequence in transgenic rice, which is suitable to treat mass materials.
以反向pcr (IPCR)为基础建立了适合于处理大量材料的克隆转基因水稻中外源基因旁侧序列的技术体系。
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