The isolation and culture of pathogenic free-living amoebae are useful in the diagnosis and research.
致病性自由生活阿米巴病原体的分离培养，对疾病诊断和病原体研究都具有重要意义。
Methods Free-living amoebae were incubated on the agaric solid medium which had been covered with Escherichia coli in control group.
方法对照组自由生活阿米巴用涂有大肠埃希菌的琼脂培养基培养。
This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
本文综述该病原体国内外报道的分离培养方法，主要包括样品处理、培养条件、虫株传代、虫株致病性检测和虫株保存方法。
This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
本文综述该病原体国内外报道的分离培养方法，主要包括样品处理、培养条件、虫株传代、虫株致病性检测和虫株保存方法。
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