Conclusion: the fusion gene expression vector was successfully constructed, and it set up the basis of inhibin gene immunization to induce multiple bear for single birth animals.
结论:高免疫原性的抑制素表达质粒的构建为利用抑制素基因免疫技术诱导单胎动物生多胎奠定了基础。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
The features of gene expression are extracted by the wavelet multi-resolution analysis, the features are classified by the support vector machines and BP neural network methods.
采用小波多分辩率分析方法提取基因表达的特征,利用支持向量机和BP神经网络方法进行分类。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
另外,杆状病毒是非复制型载体,能高效表达目的蛋白,其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的构建反义MBD1基因片段真核表达载体,为研究MBD1基因功能提供工具。
As the vector and acceptor system of gene expression in eukaryote, Drosophila cells are an important aspect of bioengineering.
果蝇细胞作为真核细胞基因表达的载体和受体系统是生物工程的一个重要研究内容。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
目的:构建基质金属蛋白酶9靶向的RNA干扰质粒载体,观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
目的:构建缺氧诱导表达载体,以介导报告基因在缺氧环境下的特异、高效表达。
Human cancer suppressor gene, protein encoded therein, expression vector containing the same, and cell transformed by the vector.
人抑癌基因,其编码的蛋白,含有其的表达载体和由该载体转化的细胞。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
目的构建人高迁移率族蛋白1(HMGB1)编码基因的表达载体,获得纯化的重组蛋白,研究其生物学功能。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
结果:将编码人单核细胞趋化蛋白—1(MCP—1)基因克隆至融合表达载体pGEX—2T中,DNA测序证实正确。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体,分析隐匿性乙型肝炎病毒S基因的突变情况,构建其原核融合蛋白表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因,构建其原核高效表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建以绿色荧光蛋白(GFP)为报告基因的酿酒酵母表达载体。
As the newly emerging vector, recombinant adenovirus is being given more attention and has become the major vector of gene therapy because of its high rate of transfection and expression.
腺病毒作为新兴的载体因其转染效率和表达效率高而日益受到重视,成为肿瘤基因治疗的主要载体之一。
Objective to construct eukaryotic expression vector of human pancreatic duodenal homeobox 1 (PDX-1) gene, and to detect its expression in NIH3T3 cell lines.
目的构建人胰十二指肠同源盒(PD X - 1)基因的真核表达载体,观察其在NIH3T3细胞中的表达。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
结论成功构建了PIG - A基因逆转录病毒载体,感染突变型k562细胞后可以提高其CD59的表达。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
目的:构建含人IL -21基因的重组腺病毒表达载体,为IL - 21基因治疗肿瘤研究奠定基础。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体,并在COS7细胞中瞬时表达。
The construction of expression vector of human ID4 promoter provides a platform for further researches on promoter activity and expression regulation of human ID4 gene.
该表达载体的构建为进一步研究人id4基因的启动子活性及表达调控奠定了基础。
Through support vector machine algorithms for gene expression data classification training, SVMs provide a effective way for analysis of gene expression data.
通过支持向量机训练算法对基因表达数据进行分类训练,为分析基因数据提供有效的手段。
Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in four different tumor cells compared with that of conventional ones.
结果:与野生型腺病毒相比,重组腺病毒能显著地提高目的基因在四种不同肿瘤细胞中的表达。
Objective: to obtain the procaryotic expression vector containing the antifreeze protein gene AFP.
目的构建可用于大肠杆菌表达系统的含抗冻蛋白基因afp的表达载体。
TK contained ALBTRS promoter sequence which probably control the targeting expression in hepatocyte of target gene TK. The enzyme cut assay of the vector LN. ALBTRS.
TK的结构中,含有ALBTRS启动子,具有在表达白蛋白的肝细胞中特异表达的潜能,载体经酶切鉴定表明结构符合要求。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体,并且在大肠杆菌中表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的从人外周血淋巴细胞中克隆出cd 81基因,构建真核表达质粒,并在COS - 7细胞中进行表达。
Conclusion: the established viral vector AdmlcGFP allows heart specific expression of a foreign gene.
结论:构建的心肌特异性表达腺病毒载体可使外源基因在心肌细胞内特异性表达。
Conclusion: the established viral vector AdmlcGFP allows heart specific expression of a foreign gene.
结论:构建的心肌特异性表达腺病毒载体可使外源基因在心肌细胞内特异性表达。
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