Objective:To obtain the hairy root of Panax ginseng (HRPG).
目的：建立人参毛状根转化体系。
Objective: To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng .
目的：研究人参毛状根生物合成熊果苷的基本条件。
Methods Hairy root of H. niger was obtained from cotyledon explants after infected with Agrobacterium rhizogenes strains A4, LBA9402, and C58C1, respectively.
方法分别用A4、LBA9402和C58C1三种发根农杆菌菌株转化莨菪子叶受体，获得发根；
The frequency of hairy root induction was closely related to the age of seedlings and explant resources.
毛状根的诱导频率与无菌苗培养天数和外植体来源部位有关。
The method of hairy root induction of plants as well as their molecular biology are reviewed.
本文综述了发根农杆菌诱导植物毛状根的方法及其分子机制。
This review deals with recent development of hairy root cultures for pharmaceuticals and points out the future ways of hairy root cultures for pharmaceuticals.
本文概述了培养毛状根生产药物研究的新成果和存在的问题，为进一步开发利用毛状根提出了可供参考的建议。
This study showed that in vitro systems establish, transformed systems establish, the choiceness hairy root lines select and the optimal culture condition of hairy roots systems establish.
主要从以下几个方面进行研究：离体培养体系的建立、遗传转化体系的建立、优良毛状根系的筛选、毛状根最佳培养条件的建立。
However, in the macroelements excessive NH4NO3 does not favor the growth of hairy root, the accumulation and release of tanshinones.
而大量元素中含量过多的NH4NO3不利于丹参毛状根的生长，同时也不利于丹参酮类成分的积累和释放。
The growth of the hairy root and the development of ginsenoside of clone R9923 were investigated and it was found that the ginsenoside content increases with the biomass of the hairy root.
研究R9923发根系的生长曲线和皂苷含量变化规律发现，随着发根生物量的增加，发根中皂苷含量积累得也越多。
And the contents of alkaloids in hairy root were determined.
莨菪发根中生物碱类含量的测定。
Phytohormones affected obviously the growth and nicotine content of the hairy root.
烟草发状根培养中，激素对发状根生长和烟碱合成有较大影响。
Phytohormones affected obviously the growth and nicotine content of the hairy root.
烟草发状根培养中，激素对发状根生长和烟碱合成有较大影响。
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