Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
探讨应用活细胞荧光染料 CFSE 检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Methods: Human peripheral blond lymphocyte (PBL) proliferation method was used and the population were classified by the length of service, classes of work and cumulated absorb dose.
方法：用外周血淋巴细胞细胞培养法对放射工作人员进行微核检测并按工龄、工种、累积吸收剂量等分类并设立对照组进行微核率的比较。
Methods: Human peripheral blond lymphocyte (PBL) proliferation method was used and the population were classified by the length of service, classes of work and cumulated absorb dose.
方法：用外周血淋巴细胞细胞培养法对放射工作人员进行微核检测并按工龄、工种、累积吸收剂量等分类并设立对照组进行微核率的比较。
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