Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
经pcr鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
METHODS ITS1 and ITS2 genes of Pogostemon Cablin from different localities were identified by PCR direct sequencing.
方法采用PCR直接测序技术对不同产地的广藿香its1和ITS2基因进行测序分析。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
To identify poliovirus isolated from feces of acute flaccid paralysis case type in gene. Isolated strains were identified by PCR-RFLP method.
对急性驰缓性麻痹病例粪便标本分离的脊髓灰质炎病毒进行型内鉴定。
Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.
结果：经PCR，酶切及测序方法鉴定，该腺病毒-整合酶嵌合系统构建成功。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive clones, of which the chromosomes were integrated with the defensin gene, were identified by the phenotype and PCR.
经表型筛选和PCR鉴定证明目的片段已稳定整合到酵母染色体基因组中。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
通过酶切、PCR及测序鉴定各重组体。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
The replicated stable results proved that two resistant genes could be identified simultaneously by using corresponding PCR primer under adaptable condition.
经反复验证，结果稳定准确，可用于在同一PCR反应体系中对两个抗病基因进行同时筛选鉴定。
Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量PCR鉴定高表达基因。
Polymorphisms was identified by restriction analysis of PCR products.
通过pcr产物的酶切证实多态性情况。
Methods the neural stem cells were isolated, identified and amplified from the cortex of aborted human embryo with 10 to 14-week gestational age, then telomerase activity was detected by PCR-ELISA.
方法分离、鉴定并扩增取自10 ~ 14周药物流产胎儿大脑皮质的神经干细胞，应用pcr - ELISA法检测神经干细胞端粒酶活性。
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常，通过DNA直接测序发现9个外显子可疑突变，但反向测序均未能证实。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
In this article all 25 Salmonella strains were accurately identified and all 3 non-Salmonella were negative by the PCR.
本文运用PCR技术检测家畜及环境中的沙门氏菌，25种沙门氏菌均获得特异性扩增，3种非沙门氏菌检测结果为阴性。
Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.
对扩增产物进行电泳鉴定，最后将PCR产物经胶回收试剂盒纯化，以备酶切。
RESULTS: The recombinant was identified by sequencing or PCR.
结果：重组子通过测序和PCR等方法分别得到验证。
The mosquitoes were firstly identified morphologically, and then Anopheles minimus A and C, An. aconitus, and An. jeyporiensis were identified by using multiplex PCR.
将捕获的蚊虫以传统方法进行形态学鉴定，然后用复合PCR法鉴别微小按蚊、乌头按蚊和杰普按蚊。
The positive bacteria strains were identified by random primer PCR.
随机引物pcr成功对阳性菌株进行了种属鉴定。
Identified by individual plant testing, analysis of RT-PCR and dot blot, this fragment was only existed in CMS cauliflower knxd612.
单株检测，RT - P CR分析，斑点杂交鉴定，确定此片段为花椰菜细胞质雄性不育系knxd612所特有。
Identified by individual plant testing, analysis of RT-PCR and dot blot, this fragment was only existed in CMS cauliflower knxd612.
单株检测，RT - P CR分析，斑点杂交鉴定，确定此片段为花椰菜细胞质雄性不育系knxd612所特有。
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