Conclusion the limbal corneal epithelial cells of rabbit could be cultivated, cryopreserved and resuscitated in vitro.
结论兔角膜缘上皮细胞可以在体外培养、冻存和复苏。
Objective to establish a method of cultivating limbal corneal epithelial cells of rabbit in vitro and to study its cryopreservation and resuscitation.
目的建立兔角膜缘上皮细胞体外培养、冻存和复苏的方法。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
目的：建立体外培养兔角膜缘上皮细胞的方法，观察其体外生长的生物学特性。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
In this research we first used HAM to culture corneal limbal epithelial cells to make HAM grafts, and succeed in curing the lack of corneal limbal epithelial cells.
本研究首次用羊膜培养表皮干细胞构建羊膜植片，并成功地治疗了角膜干细胞缺损。
In this research we first used HAM to culture corneal limbal epithelial cells to make HAM grafts, and succeed in curing the lack of corneal limbal epithelial cells.
本研究首次用羊膜培养表皮干细胞构建羊膜植片，并成功地治疗了角膜干细胞缺损。
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