The results showed that plasmolysis time, concentration of enzyme solutions, different osmoticum and incubation time significantly affected isolation of protoplasts from K326 leaves.
结果表明，质壁分离的时间、酶液浓度、渗透压及酶解时间是影响原生质体产量和质量的决定性因素。
The results showed that plasmolysis time, concentration of enzyme solutions, different osmoticum and incubation time significantly affected isolation of protoplasts from K326 leaves.
结果表明，质壁分离的时间、酶液浓度、渗透压及酶解时间是影响原生质体产量和质量的决定性因素。
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