• METHOD Whole-cell patch clamp technique was used.

    方法采用全细胞膜片技术

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  • METHODS Using cell-attached patch clamp technique.

    方法采用细胞贴附式膜片技术。

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  • Methods Whole-cell patch clamp technique was used.

    方法采用全细胞膜片钳制技术。

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  • METHODS Whole cell recording by patch clamp technique.

    方法膜片细胞记录

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  • METHODS Patch clamp technique with whole cell configuration.

    方法膜片钳制技术细胞记录模式。

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  • Methods: Patch clamp whole cell recording technique was used.

    方法膜片细胞电流记录方法

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  • Methods The whole-cell patch clamp with current clamp was used.

    方法采用全细胞膜片技术,进行电流钳制。

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  • METHODS The patch clamp whole cell recording technique was used.

    方法采用膜片细胞式记录技术。

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  • Methods:Using nystatin perforated patch clamp recording configuration.

    方法采用制霉菌素穿孔膜片技术。

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  • Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.

    方法采用全细胞膜片技术,记录通道电流

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  • The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.

    膜片技术广泛应用极大推动了人们离子通道认识

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  • Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.

    方法采用改进分离法分离单个细胞膜片细胞记录法进行鉴定。

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  • The patch clamp technique bring about new light set in studying on mechanism of atherosclerotic development.

    膜片技术动脉粥样硬化发病机理研究带来了新的亮点。

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  • The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.

    常规酶解法分离成年大心室肌细胞,采用全细胞膜片技术观察U50,488H对电流作用。

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  • CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.

    结论膜片技术使探讨烟碱受体拮抗药作用位点成为可能。

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  • Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.

    方法通过膜片手段测量分化SHSY5Y细胞离子通道电流

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  • The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.

    膜片技术记录心肌细胞通道电流首先必须获得单个心肌细胞。

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  • Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.

    结论成功建立了一种简单快速适用膜片钳技术大鼠皮层神经元急性分离改良方法

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  • Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.

    方法膜片细胞记录测量培养大鼠海马神经元钾离子电流

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  • Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.

    方法离体培养A549细胞上,膜片钳全细胞模式记录通道电流

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  • Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.

    方法应用细胞膜片电流箝制技术,对急性分离大鼠DRG细胞进行电流箝制。

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  • METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.

    方法培养大鼠海马神经元膜片细胞记录通道电流

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  • Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.

    根据检测目的不同,微电极主要应用可以分为离子选择微电极检测技术膜片技术。

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  • Patch clamp technique and its application in Cardiovascular pharmacology, especially meticulous expounded ion channel about myocardium Were Summarized.

    综述膜片技术心血管药理学中的应用特别是与心肌有关的离子通道做了细致地阐述

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  • Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.

    方法运用细胞膜片技术记录持续性钠电流观察缺氧-复氧模型变化

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  • A combination of mechanical and enzymatic isolation method is described for acutely isolating rat hippocampal neurons suitable for patch clamp recording.

    结合消化机械分离方法,探讨一种适用膜片记录的大白鼠海马神经元急性分离技术。

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  • Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.

    膜片技术记录离子通道离子电流来反映细胞膜上单一多个的离子通道分子活动的技术。

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  • The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.

    膜片技术一种先进电生理技术生命科学研究得到了广泛应用。

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  • In patch clamp experiment, the formation of giga-ohm seal is a critical step, which is usually fulfilled through controlling the air pressure within pipette and the holder.

    膜片实验中欧封接形成至关重要的步骤一般通过控制电极与电极夹持器气体压力完成

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  • Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.

    方法用膜片记录仪体外培养不同时期记录大鼠海马神经元GABA-A受体离子通道细胞通道电流电导变化

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