Methods:Using nystatin perforated patch clamp recording configuration.
方法：采用制霉菌素穿孔膜片钳技术。
A combination of mechanical and enzymatic isolation method is described for acutely isolating rat hippocampal neurons suitable for patch clamp recording.
结合酶消化和机械分离的方法，探讨一种适用于膜片箝记录的大白鼠海马神经元的急性分离技术。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Method: With conventional Patch Clamp whole-cell recording technique.
方法：采用常规膜片钳全细胞记录技术。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
METHODS Whole cell recording by patch clamp technique.
方法膜片钳全细胞记录。
METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2 + current in single cardiac myocyte.
方法：用全细胞膜片箝记录心室肌细胞的L -钙电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
An stable method for human myocytes ion-channel patch-clamp recording were established.
应用此系统我们建立了稳定的人心肌细胞离子通道膜片钳记录方法。
METHODS: Whole cell patch-clamp recording techniques.
方法：全细胞膜片箝技术。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
The methods of isolation and primary cultivation of hippocampal pyramidal neurons of SD rats improved the growth of neurons and so might be used in patch-clamp recording.
表明本分离及原代培养方法，有益于大鼠海马锥体神经元生长，并适用于膜片钳记录。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法：采用细胞贴附式的膜片钳技术。
Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.
定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。
The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
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