Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.
现行的多元聚合酶链反应(PCR)技术最多只能在50种生物内进行一次检测。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
The diagnosis is supported or confirmed by growing the bacteria from specimens of spinal fluid or blood, by agglutination tests or by polymerase chain reaction (PCR).
通过脊髓液或者血液标本培养出细菌，做凝集试验或者聚合酶链反应(PCR)实验，会支持或确认诊断结果。
Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.
每个实验室都有一台实时多聚酶链式反应机，有一个实验室有两台这样的机器。
Polymerase chain reaction (PCR) assay.
聚合酶链反应(PCR)测定。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
方法：采用聚合酶链反应(PCR)技术，检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
Objective To study the value of multiplex polymerase chain reaction (PCR) in diagnosing herpes virus infection of eye bank cornea donor.
目的应用多引物pcr方法快速诊断眼库供体角膜疱疹病毒感染，探讨角膜移植术后移植衰竭和疱疹病毒感染的关系。
Objective:To evaluate the diagnostic value of polymerase chain reaction (PCR), culture, Semar acid fast staining, fluorescent staining in tuberculosis.
目的：评价聚合酶链反应（PCR） ，细菌培养，抗酸染色，荧光染色对结核病的诊断价值。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
需要使用包括聚合酶链反应（PCR），琼脂糖凝胶电泳，探针的标记与检测等生物工程技术。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Polymerase Chain Reaction (PCR) technique has been widely used in detecting different hepatitis viruses in laboratory, because of its high specificity and sensitivity.
聚合酶链反应(PCR)技术用于病毒核酸的检测具有灵敏度高，特异性强等优点，已广泛用于各型肝炎病毒的实验室检测。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
A rapid method for detection and identification of equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) was developed using polymerase chain reaction (PCR).
聚合酶链反应(PCR)可作为一种快速检测并鉴别马疱疹病毒1型(EHV 1)和马疱疹病毒4型(EHV 4)的诊断方法。
Polymerase chain reaction (PCR) extensively utilized for research about oral bacteria recently.
近年来聚合酶链反应(PCR)已应用于口腔细菌的研究。
Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA测序来加以鉴定。
Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
The polymerase chain reaction (PCR) machine is a basic instrument in molecule biology.
聚合酶链式反应仪(PcR仪)是分子生物学的基本仪器。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods Polymerase chain reaction(PCR).
方法聚合酶链反应。
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
Mehtods a case control study was carried out and polymerase chain reaction (PCR) technique was used to identify GSTM1, GSTT1 genotype in 89 cases of primary gastric cancer and 94 controls.
方法采用病例对照分子流行病学研究方法和聚合酶链反应技术，检测89例原发性胃癌病例和94例对照GSTM 1和GSTT1基因型。
Objective To explore simple method for cloning the products amplified with polymerase chain reaction (PCR).
目的探讨克隆聚合酶链反应(PCR)扩增产物的简便方法。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
A 40-base polymorphic repeat sequence located in the 3 '-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
将位于多巴胺运输器基因3'端未转译区段的40 -碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。
A 40-base polymorphic repeat sequence located in the 3 '-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
将位于多巴胺运输器基因3'端未转译区段的40 -碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。
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