Conclusion: The MAGE-3 fragment can be used in eukaryotic and prokaryotic gene expression.
结论该片段可用于真核及原核表达。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Horizontal gene transfer is an important evolutionary mechanism in prokaryotic genome evolution and has been widely studied.
在原核生物的进化过程中，基因的横向转移是一个重要的进化机制。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
ConclusionThe augementer of liver regeneration gene was cloned from the liver tissue of 2 week old SD rat and the protein encoded by the gene was expressed in the prokaryotic cells.
结论从2周龄大鼠肝组织中成功克隆肝再生增强因子基因，并获得了原核细胞高效表达。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体，并且在大肠杆菌中表达。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective to construct the prokaryotic expression vector of human neuronal pentraxin 2 (NPTX2) gene, induce the expression of the recombinant fusion protein in e.
目的在大肠埃希菌中表达人神经元正五聚蛋白2 (NPTX2)，并对该重组蛋白进行纯化、鉴定。
In this study, the Aer toxin gene was cloned and expressed in prokaryotic system and the antigenicity of its recombinant protein was analyzed.
该领域国外研究热点在嗜水气单胞菌毒素的基因组结构、相关毒力因子基因克隆和序列比较以及毒素蛋白的表达和致病机理等方面。
We use gene recombination technology to build the prokaryotic MGC5306 expression system, and we have successfully expressed MGC5306 (98-204aa).
我们利用基因重组技术构建了MGC5306的原核表达体系，并成功表达了MGC5306 (98- 204aa)。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
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