Methods The method of luciferase reporter gene was used.
方法采用荧光素酶报告基因方法。
Both proteins are then expressed in yeast cells that have a reporter gene.
之后让两种蛋白都在具有一种报告基因的酵母细胞中表达。
As a reporter gene, GFP has been used in a variety of biological researches.
GF P基因作为报告基因已被广泛应用于各种生物的研究中。
The AR) reporter gene assay is useful in screening androgenic effect of chemicals.
本研究建立的AR报告基因试验可作为化学物雄激素活性筛选的理想方法。
The reporter gene was used to study the mutation influencing the activity of promoter.
对发现的突变，进行荧光素酶报告基因瞬时表达研究。
Luciferase reporter gene technique plays an important role in high throughput screening.
荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
At present, the key problem is focused on the selection of what kind of reporter gene probe would be.
当前，报告基因显像的焦点是选择何种报告基因探针进行基因显像。
After gene transfection, GH3 cell specifically expressed reporter gene and killed specifically by therapy gene.
基因转染后，GH 3细胞特异性表达报告基因，并被治疗基因特异性杀伤。
To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.
为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。
To develop an androgen receptor (AR) reporter gene assay in which the reporter gene is chloramphenicol acetyltransferase (CAT).
建立以氯霉素乙酰化酶(CAT)为报告基因的雄激素受体(AR)报告基因试验。
In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.
核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
以含雌激素反应元件的荧光素酶(ERE -LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
The reporter gene vector driven by SOD2 promoter will provide an experimental tool for the further study on the regulatory mechanism of the SOD2 expression.
该启动子的成功克隆和其报告基因载体的构建，为研究SOD2的基因表达调控机制提供了重要基础和工具。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
将其与GUS报告基因融合在一起，构建了植物表达载体，并由农杆菌介导法导入水稻品种‘中花11’中。
In the paper we review several strategies to monitor the gene therapeutic efficacy by certain receptor gene as reporter gene transferred together with therapeutic ge...
本文主要综述了受体基因作为报告基因与治疗基因共同转基因并通过放射性核素显像监测表达的方法。
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
目的以RNA依赖性腺嘌呤脱氨酶（ADAR1）为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
Reporter gene imaging is an important imaging technique in molecular imaging, which can image various biological and genetical processes. It has a potential in clinical application.
报告基因显像技术是分子影像学中重要的显像技术，其可对多种不同的生物学和分子遗传学过程进行显像，有望更快地应用于临床。
Objective to study the effect of ionizing radiation on the expression of liposome mediated GFP reporter gene drived by EGR - 1 gene promoter and CMV promoter, respectively in hepatoma 7402 cells.
目的研究电离辐射调控脂质体介导的EGR-1基因启动子、CM V启动子驱动的GF P报告基因在人肝癌7402细胞内的表达。
Bejerano and colleagues took the switch information from a chimpanzee's genome and essentially "hooked it up" to a reporter gene, a gene whose effects scientists can track as an organism develops.
Bejerano和同事们从黑猩猩的基因组提取开关信息，把它们附加到一组可以报告的基因上，当生物发育时，科学家们可以跟踪这组基因的影响。
They also recorded gene expression in real - time using a bioluminescent reporter of gene activity.
同时他们采用基因活性生物发光法记录了基因的实时表达情况。
Finally, there is an increase in the expression of a reporter transgene driven by a neuronal-specific promoter and a decrease of gene expression using a glial specific promoter in VPA-treated cells.
最后，出现了由神经元特异启动子促发的转基因的报道基因表达的增加，同时出现了依赖于胶质特异启动子的基因表达的下降。
Finally, there is an increase in the expression of a reporter transgene driven by a neuronal-specific promoter and a decrease of gene expression using a glial specific promoter in VPA-treated cells.
最后，出现了由神经元特异启动子促发的转基因的报道基因表达的增加，同时出现了依赖于胶质特异启动子的基因表达的下降。
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