This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
本研究旨在构建稳定表达T7RNA聚合酶的猪睾丸细胞系(ST细胞系)，用于猪瘟病毒等的反向遗传操作。
The L protein, the major component of viral RNA dependent RNA polymerase is associated with the protein kinase for phosphorylation of NS protein.
病毒的L蛋白除以前报道的具有RNA聚合酶活力外，也具有蛋白激酶的活力。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
RNA polymerase in RNA virus is different from host cells restrict it's high and early expression. The restrict is moderate compared with other virus genes.
RNA病毒的聚合酶基因总体上和宿主密码子使用类型不一致，限制了聚合酶基因的及早和过高表达，但其密码子使用频率对聚合酶的限制是适中的。
Actin was also reported to co-purify with RNA polymerase II and to be required for efficient in vitro transcription by RNA polymerase II.
肌动蛋白还能与RNA聚合酶II共纯化，对RNA聚合酶II体外的有效转录起到作用。
Actin was also reported to co-purify with RNA polymerase II and to be required for efficient in vitro transcription by RNA polymerase II.
肌动蛋白还能与RNA聚合酶II共纯化，对RNA聚合酶II体外的有效转录起到作用。
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