But neighbourhoods have also changed, with the local services such as post offices that tended to form the heart of old communities on the decline.
但邻里关系也发生了变化，当地的服务设施比如本应该建在老年社区中心的邮局的数量也减少了。
After 1815 this older form of manufacturing began to give way to factories with machinery tended by unskilled or semiskilled laborers.
1815年后，这种古老的生产方式开始让位于那些由无技能或半熟练工人操作机器的工厂。
When the cells at high confluence(>50%) or cultured with low concentration of serum(2% FBS), they tended to fuse to form myotubes.
细胞在高汇合度（>50％）或低血清（2％FBS）培养时，极易融合成肌管或肌细胞链；
When the cells at high confluence(>50%) or cultured with low concentration of serum(2% FBS), they tended to fuse to form myotubes.
细胞在高汇合度（>50％）或低血清（2％FBS）培养时，极易融合成肌管或肌细胞链；
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