A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库,它可以用来筛选结合特定抗体的蛋白。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
根据计算机克隆的ZNF322基因序列设计引物,从人类胚胎心脏文库中克隆了ZNF322基因。
RESULTS: human NS5ABP37 was screened and cloned from human liver cDNA library by yeast-two hybrid system 3. The murine NS5ABP37 was deduced by bioinformatics methods.
结果:通过酵母双杂交技术获得了人ns5abp37的编码基因。利用生物信息学技术确定了小鼠ns5abp37的基因序列。
Objective To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.
目的分离晕船适应大鼠下丘脑差异表达基因片段,以探讨晕船适应机理。
The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.
将文库菌落印迹至尼龙膜,分区培养提取质粒DNA ,建立基因池,并分别转染NIH/3T3细胞。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
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