The analysis result showed that the contents of total alkaloids in hairy roots were higher than explants and calli.
检测结果表明，毛状根中的总生物碱含量高于长春花的原植株和愈伤组织。
The contents of total alkaloids extracted by two methods were 2.85% and 1.86 respectively.
酶浸法和氯仿法提取马钱子生物总碱的产率分别为：2.85%、1.86%。
Correlation analysis showed that the relationship between the contents of total alkaloids and galanthamine was positive in the same place, the correlation coefficient was 0.601.
同一地区样品中总生物碱与加兰他敏的含量呈一定的正相关，相关系数为0.601，但并不显著。
METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively.
方法：采用酸性染料比色法测定附子总生物碱含量，反相高效液相色谱法测定双酯型生物碱含量，蒽酮-硫酸法测定多糖含量。
METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively.
方法：采用酸性染料比色法测定附子总生物碱含量，反相高效液相色谱法测定双酯型生物碱含量，蒽酮-硫酸法测定多糖含量。
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