In current, different kinds of methods were widely used in the animal model, such as the wild type, congenital defect and gene modification.
目前野生型、先天缺陷型、基因修饰等动物模型被广泛应用在这一研究领域。
Female wild-type mice on an ethanol-rich diet had 4 times as much alanine aminotransferase (ALT), indicating liver injury, than mice lacking the osteopontin gene.
雌性野生种小鼠的丙氨酸转氨酶是基因敲除鼠的4倍，这提示肝损伤的存在；
But wild type GFP and EGFP are not ideal to detect the transient changes of gene expression regulation.
但野生型GFP及增强型GFP等因半衰期长，稳定性强，对反映一过性转录表达调控的变化不甚理想。
Conclusion Transfection of wild-type PTEN tumor suppressor gene might significantly reduce metastatic ability of human highly metastatic mucoepidermoid carcinoma cells.
结论转染野生型PTEN抑癌基因能降低人高转移性黏液表皮样癌细胞转移能力。
Objective To study effects of the exogenous wild-type PTEN tumor suppressor gene on in vitro adhesion, migration and invasion of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.
目的探讨外源野生型PTEN抑癌基因对人高转移性黏液表皮样癌细胞系m 3sp2体外黏附、迁移和侵袭特性的影响。
Recessive allelic variations were investigated at 3 microsatellite (SSR) sites within the o2 gene by using 14 inbred o2 lines and a wild-type line in maize.
用14个O2玉米自交系和1个普通玉米自交系研究玉米o2基因内3个微卫星(SSR)位点的隐性等位变异。
Objective: the wild type FHIT gene was transfected into the human gastric cancer cell line MGC-803 in which the FHIT gene had been totally lost so as to investigate its effect on Adriamycin (ADM).
目的：将FHIT基因导入该基因表达缺失的人胃癌细胞株mgc- 803，探讨胃癌中FHIT基因表达对阿霉素(adm)敏感性的影响。
Conclusion the exogenous wild-type PTEN gene has significant effects of anti-adhesion, anti-migration, and anti-invasion on the highly metastatic mucoepidermoid carcinoma cell line M3SP2.
结论外源性pten抑癌基因对高转移性黏液表皮样癌细胞系m 3sp2体外黏附、迁移和侵袭具有抑制作用。
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
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