Aim to study the mutual influence of membrane current induced by receptors with diverse coupling modes in Xenopus oocytes.
目的研究不同偶联模式的兴奋性氨基酸受体所介导非洲爪蟾卵母细胞跨膜电流的相互影响。
Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.
定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。
Methods: the two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles.
方法:在去除滤泡膜的非洲爪蟾卵母细胞上,建立双电极电压钳记录技术,并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。
Methods: the two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles.
方法:在去除滤泡膜的非洲爪蟾卵母细胞上,建立双电极电压钳记录技术,并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。
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