等电聚焦可以用为另一指标。
固相pH梯度等电聚焦是国际上80年代的新型电泳技术。
The mentioned immobilized PH gradients iso-electrofocusing is a electrophoresis techniquedeveloped in 80' s.
采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。
Haemoglobin polymorphism of Chinese miniature horses was examined by agarose gel isoelectric focusing.
定量后,分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35;
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
和传统的凝胶技术相比,毛细管等电聚焦(CIEF)是一种蛋白质的快速分离技术。
Capillary isoelectric focusing (CIEF) is a more rapid technique for separating proteins that the traditional gel-based technique.
讨论了毛细管等电聚焦中所涉及的问题,如分离机理、电渗、迁移方法、检测器及其应用。
Separation mechanism, manipulation of electroosmosis, mobilization methods, detectors and some problems associated with the determination of samples are discussed.
单花粉蛋白等电聚焦结合显微操作技术对于遗传育种和发育生物学研究具有重要的应用前景。
The technique of IEF of single pollen grains and micro-operation provides a new and quick screening test applied to plant genetic breeding and developmental research.
单花粉蛋白等电聚焦结合显微操作技术对于遗传育种和发育生物学研究具有重要的应用前景。
The technique of IEF of single pollen grains and micro operation provides a new and quick screening test applied to plant genetic breeding and devel…
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离,然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法,分析了栗属种的PGI同工酶的遗传。
Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method.
SDS聚丙烯酰胺凝胶电泳测定分子量为64,000。等电聚焦电泳测得等电点在4.2左右。
Its molecular weight is 64,000 and isoelectric point around pH 4.2 as determined by SDS-PAGE and isoelectric focusing analysis respectively.
本文用聚丙烯酰胺等电聚焦(PAGIEF)对来自6个目、15个科、20种动物毛发角蛋白组分进行了研究。
The keratin compositions of the human and animal hairs belonging to 6 orders, 15 families and 20 species was studied by polyacrylamide gel isoelectric focusing (PAGIEF).
方法:采集33例正常人,26例牙周炎患者全唾液,运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.
用等电聚焦电泳法测得其等电点约为7.2。结论:从栖热菌中提取的耐热乳糖酶的理化性质不同于其它来源的乳糖酶。
Its pI was about 7.2. Conclusion:The thermostable lactase from strain of thermus sp A3 was different from lactases from other microorganism.
通过对聚丙烯酰胺薄层平板等电聚焦电泳的系统研究,建立了一套适合于分析明胶等电点分布的薄层平板等电聚焦电泳技术。
In this paper the isoelectrofocusing on polyacrylamide thin slab-bed was studied systematically and the method suited for analyzing photographic gelatin was established.
结论:SDS - PAGE和等电聚焦电泳均可获得燕窝蛋白质的特征性电泳图谱,用于鉴别不同品种燕窝及掺伪燕窝是可行的。
Conclusions: SDS-PAGE can be used in identification of EBN and IEF can be used to produce characteristic pattern of EBN by separating proteins.
从酯酶同工酶等电聚焦电泳得出,ABM-R种群的E7、E13和E15同工酶活力显著提高是导致ABM- R种群酯酶活力提高的主要原因。
Esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in ABM R was due to the increased activities of E7, E13 and E15 isozymes.
在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
Isoelectric focusing of STFC in thin-layer polyacrylamide gel obtained only one band as shown by Coomassie Blue staining.
在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
Isoelectric focusing of STFC in thin-layer polyacrylamide gel obtained only one band as shown by Coomassie Blue staining.
应用推荐