[目的]探讨人胰岛素样生长因子(human insulin-like growth factor-1,hIGF-1)基因在退变椎间盘中的表达及对椎间盘中Ⅱ型胶原表达的影响。[方法 .
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1 主要实验材料、仪器及设备 2.1 hIGF-1基因在椎间盘中的表达 1 材料与方法 ［目的］探讨人胰岛素样生长因子（human insulin-like growth factor-1,hIGF-1)基因在退变椎间盘中的表达及对椎间盘中Ⅱ型胶原表达的。
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人胰岛素样生长因子I human insulin like growth factor
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
目的构建人胰岛素样生长因子- 1的逆转录表达载体，建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
人胰岛素样生长因子- 1是一种对机体生长和代谢具有广泛作用的分泌蛋白，与机体的营养调节密切相关。
As a kind of secreted protein, the insulin-like growth factor-1 (IFG-1) has diverse effects on growth and metabolism, and contributes to the nutritional regulation of the body.
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