...分型含量测定： (1) 将标本Ⅰ液和Ⅱ液上样，经聚丙烯酰胺凝胶电泳(Sodium dodecyl sulphate polyacrylamide gel electrophoresis, SDS-PAGE)证实，有α2(Ⅰ)、α1(Ⅰ)、β和γ区带的为Ⅰ型胶原；有α1(Ⅱ)、β和γ区带为Ⅱ型胶原。
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经聚丙烯酰胺凝胶电泳分析，细胞的酯酶同功酶带为12条，其中有6条主带。
The esterase isozyme exhibited 12 isozyme bands by PAGE with 6 major bands.
纯化后的酶液经聚丙烯酰胺凝胶电泳，分别采用银染色和多酚氧化酶活性染色均显示一条电泳带。
The result from polyacrylamide gel electrophoresis of purified enzyme indicated that only one band was found using silver stain and PPO activity stain, respectively.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
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