(5)测量:用酶标仪测量波长为595nm 时每孔的光吸收值(A),计算细胞的存活率(Survival Rate,SR)和IC50(抑制肿瘤细胞生长达到50%时的药物浓度),所有实验重复三次。
基于1个网页-相关网页
采用四甲基噻唑兰(MTT)比色法测细胞毒性作用,并计算细胞的存活率利治疗指数(TI)。
MTT method was used to evaluate the cytotoxicity, and the cell viability and therapeutic index (ti) was calculated.
四甲基偶氮唑蓝比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。
Cell viability was examined by methyl thiazolyl tetrazolium (MTT) assay and 50% inhibitive concentration (IC50) of cisplatin was examined also.
应用推荐