胎盘贴壁细胞的生物学特性-其它医学论文-中国论文联盟 Keywords】 placenta; adherent cells; stem cells [gap=400]关键词】 胎盘；贴壁细胞；干细胞
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无菌吸管 离心管 培养瓶 无菌吸管/离心管 离心管/培养瓶 3. 步骤： 步骤： 3.1. 附着型胞 附着型胞(adherent cell) 3.1.1. 吸掉旧培养液。
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heart derived adherent cell 心脏黏附细胞
adherent cell sorting 粘附细胞的分选
fetal lung-derived adherent cell 肺贴壁细胞 ; 胎肺贴壁细胞
human placental derived adherent cell 人胎盘源贴壁细胞
non-adherent cell 非黏附细胞
splenic adherent cell 脾粘附细胞
adherent cell monolayer 单层贴壁细胞
marrow-derived adherent cell 骨髓衍生粘连细胞
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。
Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.
结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞，是一种比较理想的分离培养方法。
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