Different fluorescent colors from pine wood nematode(Bursaphelenchus xylophilus) was observed after staining with 4,6-diamidino-2-phenyiindole 2 hci(DAPI)or aniline blue or acridine orange.

利用4，6-联脒-2-苯基吲哚（DAPI）、苯胺蓝、吖啶橙等染料对松材线虫进行染色处理，荧光显微镜观察发现松材线虫经不同的荧光染料染色后，产生不同的荧光效果，且荧光效果均强于其自发荧光。

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