...究_好大夫在线 关键词】 角膜缘上皮细胞， 低温保存， 细胞培养， PCNA [gap=1531]Key wordsl Corneal limbal epithelial cells； Cryopreservation； Cell culture； PNCA ...
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...究 专 业：眼科学 硕士研究生：刘敬波 导 师：王智崇 教授 摘要 目的：探讨人角膜缘上皮细胞（corneal limbal epithelial cells,CLECs）体外分离、纯化、建系的条件；探讨结合异种角膜基质板层，借助旋转培养形成的气液界面，进行板层生物角膜构...
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Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
In this research we first used HAM to culture corneal limbal epithelial cells to make HAM grafts, and succeed in curing the lack of corneal limbal epithelial cells.
本研究首次用羊膜培养表皮干细胞构建羊膜植片，并成功地治疗了角膜干细胞缺损。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
目的：建立体外培养兔角膜缘上皮细胞的方法，观察其体外生长的生物学特性。
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