CONCLUSION: MTX induced-increase of recombinant antibody production in CHO cells can be increased by weakening the expression of DHFR gene.
结论：弱化表达载体中DHFR的表达，可提高MTX对工程抗体表达的增加效果。
The molecular mechanism of resistance to the antifolates has been well characterisied and is due to structural changes in the target enzymes resulting from the point mutations of DHFR or DHPS gene.
抗叶酸类抗疟药的抗药性机制已基本搞清，与其作用靶酶二氢叶酸还原酶或二氢蝶酸合成酶基因点突变相关。
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