Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
DNA were extracted from the peripheral blood, then TG sequence in second intron of IL - 4 was amplified by PCR, the polymorphism of that segment were then detected through 8% polyacrylamide gel.
首先从实验对象的外周血中提取DNA, pcr扩增il - 4的第二个内含子中的一段TG重复序列，然后用8%的聚丙烯酰胺凝胶电泳检测这段基因的多态性。
Through the downloading of the rice sequence and the data analysis, a DNA polymorphism database between japanica and indica was constructed.
我们利用网上公布的水稻基因组的测序结果，经过数据处理和分析，构建了一个水稻亚种粳稻和籼稻间的DNA多态性数据库。
应用推荐