毛细管等电聚焦(Capillary Isoelectric Focusing CIEF),是两性电解质在分离介质中形成pH梯度,使各种不同具有等电点的蛋白质在电场作用下,按照这一梯度迁移到等电点的位置,由此形成一...
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1985年,Hjerten[28】将等电聚焦电 泳方法引进CE分离,使毛细管等电聚焦(capillary isoelectric focusing,C LEF) 技术在蛋白质分离中成为一个强有力的微柱分离分析工具。
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药物分析专业词汇 (2) ... 毛细管凝胶电泳capillary gel electrophoresis,CGE 毛细管等电聚焦capillary isoelectric focusing,CIEF 毛细管等速电泳capillary isotachorphoresis,CITP ...
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和传统的凝胶技术相比,毛细管等电聚焦(CIEF)是一种蛋白质的快速分离技术。
Capillary isoelectric focusing (CIEF) is a more rapid technique for separating proteins that the traditional gel-based technique.
讨论了毛细管等电聚焦中所涉及的问题,如分离机理、电渗、迁移方法、检测器及其应用。
Separation mechanism, manipulation of electroosmosis, mobilization methods, detectors and some problems associated with the determination of samples are discussed.
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离,然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
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