DNA分子提取得到以后,需要通过电泳技术来检测其数量和质量。自从琼脂糖和聚丙烯酰胺凝胶被引入核酸研究以来,按相对分子质量大小分离DNA的凝胶电泳技术,已经发展成为一种分析鉴定DNA分子的重要实验手段。琼脂糖或聚丙烯酰胺凝胶电泳是基因操作的核技术之一,它能够用于分离、鉴定和纯化DNA片段。该技术操作简单而迅速,已经成为许多通用的分子生物学研究方法,如DNA重组、DNA核苷酸序列分析、DNA限制性内切酶分析及限制性酶切作图等的技术基础。
:以凝胶为介质,在电场作用下分离蛋白质或核酸的分15,凝胶电泳(gel electrophoresis)离纯化技术。16,SDS-聚丙烯酰氨凝胶电泳(SDS-PAGE):在去污剂十二烷基硫酸钠存在下的聚丙烯酰氨凝胶电泳。
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改变性别梯度凝胶电泳(DGGE)是一种能够将具备单碱基区别的DNA离合的办法。其原理是:当双链DNA在改变性别梯度凝胶中施行到与DNA改变性别温度对应完全一样的位...
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差异凝胶电泳 差异凝胶电泳(DIGE):应用两种不同的荧光染料分别标记不同的蛋白质样品后等量混合,2-DE 后置于荧光 差异凝胶电泳 显微镜下观察,如同一斑点可见两种荧...
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...的是一样的电泳的话,无所谓啊顶多盐多一点点,电流会高一点点而已 脉冲凝胶电泳和毛细管电泳仪有什么区别脉冲凝胶电泳(PFGE,Pulsed Field Gel Electrophoresis)是一种分离大分子DNA的方法。
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脉冲场凝胶电泳 pulsed field gel electrophoresis ; PFGE ; PFGE'S Agarose ; Resistance
温度梯度凝胶电泳 Temperature gradient gel electrophoresis ; TGGE ; PCR-TGGE
变性梯度凝胶电泳 DGGE ; denaturing gradient gel electrophoresis ; denaturing gel gradient electrophoresis ; Degenerative gradient gel electrophoresis
聚丙烯酰胺凝胶电泳 [物化] polyacrylamide gel electrophoresis ; PAGE ; PAGE - Polyacrylamide gel electrophoresis ; SDS-PAGE
毛细管凝胶电泳 CGE ; capillary gel electrophoresis
单细胞凝胶电泳 SCGE ; single cell gel electrophoresis technique ; single cell microgel electrophoresis
脉冲电场凝胶电泳 PFGE ; pulsed-field gel electrophoresis ; [化学] pulse alternative field gel electrophoresis
圆盘凝胶电泳 disc gel electrophoresis
单细胞凝胶电泳技术 SCGE ; Single cell gel electrophoresis ; single cell microgel electrophoresiss
Leptin receptor was examined with agarose gel electrophoresis at last.
72℃延伸7分钟,反应结束后琼脂糖凝胶电泳检测。
参考来源 - 瘦素对胰岛素合成、分泌和信号转导的影响To confirm the ctxAB gene, the amplified product was analyzed by agarose gel electrophoresis detection and sequencing.
裂解细菌,获取扩增产物,琼脂糖凝胶电泳检测和测序的结果表明扩增产物是ctrAB基因的序列。
参考来源 - 鲍鱼养殖水系中霍乱弧菌基因水平转移的初步研究·2,447,543篇论文数据,部分数据来源于NoteExpress
经凝胶电泳分析,发现四对实验对象中有两对的酶的氨基酸序列略有差异。
Analysis by gel electrophoresis revealed slight differences in amino acid sequence of the enzymes of two of the four pairs.
分子量通过三辛凝胶电泳确定。
The molecular weight was determined by tricine gel electrophoresis.
用琼脂糖凝胶电泳检测两类卵的颗粒细胞凋亡。
Granulosa cell apoptosis was detected by agarose gel electrophoresis.
Well the way to find it is by using a process called electrophoresis and Southern blotting.
找到二者差异的方法称作电泳和,琼脂糖凝胶电泳印迹
If this was a sickle patient, so they had this gene instead it wouldn't get cut and when I went to look for that presence of that gene on this gel, it would appear as one large segment instead of a large one and a smaller one.
而如果是个镰状红细胞贫血的病人,他们的基因是不会被限制酶切割的,因此他们基因的凝胶电泳结果就不会呈现,一大一小的结果,而只有一个大的片段
I'm sure some of you have dealt with electrophoresis gels in the past and it's really just a slab of something like Jell-O where you can put a sample at one end of the Jell-O, your sample of fragmented DNA.
我相信在座肯定有些已经接触过电泳凝胶,真的挺像一块板状果冻的,你可以把一段样本放在"果冻"的一端,你的DNA片段样本
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