...0μl细胞悬液与含1%RNA酶的Tis-HCl缓冲液混匀共同孵育10min,加入碘化丙啶(PI)和异硫氰酸荧光素(flucresceinisothiocyanate,FITC)标记的磷脂酰结合蛋白(AnnexinV)各5μl混匀,避光37℃孵育30min,FCM(FACSca...
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用破伤风毒素和异硫氰酸荧光素标记马抗破伤风血清可以特异地显示体外培养的神经细胞。
Tetanus toxin and FITC labelled horse anti-tetanus toxoid serum were applied to show the plasma membrane of cultivated chicken embryonic cerebral cortical cells.
荧光定量方法的建立:用异硫氰酸荧光素成功对白蛋白和纤维蛋白原进行了荧光标记。
Establish the quantitive fluorimetry: Bovine serum albumin (BSA) and fibrinogen (Fg) was successfully labeled with FITC.
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