活菌计数法又称间接计数法。活菌计数法是在一定浓度的定量药物内加入定量的试验菌,经过一定时间培养后,取样进行活菌计数。活菌计数是将定量的药物与试验菌作用后的混合液稀释后加入琼脂培养基,制成平板,培养后数平板上形成的菌落数。直接计数法测定到的是死、活细胞总数,而间接计数法测得的仅是活菌总数。这类方法所得的数值往往比直接计数法测得的数值小。
采用滤纸片法、液体培养法和活菌计数法,研究了蜜环菌菌索提取液对常见微生物的抑制作用。
The antibacterial action of the rhizomorph from the Armillaria mella was studied by the methods of AGAR diffusion, liquid cultivation and surviving bacteria count.
采用管碟法、试管法、活菌计数法对鱼腥草等中药注射液体外抗菌活性进行测定,结果表明采用活菌计数法,用原液可以测出用管碟法所不能测出的中药抑菌作用。
Tube plate method, tube dilution method and viable count method were used to determine the inhibitory activity of traditional Chinese medicine injection in vitro, such as Houttuynia Cordata Thunb etc.
介绍一种旋转表面涂布活菌计数方法,具有螺旋平板法及表面涂布法的优点。
A spiral and surface spread plate method (SSSPM) for counting viable bacteria is introduced.
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