在高K+刺激下的PC12细胞中,我们运用全内反射荧光显微镜(total internal reflection fluorescence microscopy,TIRFM)技术,通过VAMP2-pHluorin和VAChT-TDimer2双色荧光成像的方法跟踪类突触小囊泡...
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这里用全内反射荧光显微镜来进行观察，就可以把后者发出的背景荧光过滤掉。
Use a total internal reflection fluorescence microscope to observe, the latter can be sent to filter out the background fluorescence.
单分子灵敏度是通过全内反射荧光显微镜(TIRF)实现的，用一个精心设计的激光产生快速衰减的倏逝波。
Single molecule sensitivity is achieved through total internal reflectance fluorescence (TIRF) microscopy, in which a precisely angled laser generates a fast-decaying, evanescent wave.
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