...esis,2-DE)基本原理是,在相互垂直的两个方向上,利用蛋白质等电点和分子量的差异,运用等电聚焦电泳(isoelectric focusing,IEF)和sDs一聚丙烯酰胺凝胶电泳将蛋白质分离开来[1610固相pH梯度(immobilizedpH gr...
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...,2-DE)基本原理是,在相互垂直的两个方向上,利用蛋白质等电点和分子量的差异,运用等电聚焦电泳(isoelectric focusing,IEF)和sDs一聚丙烯酰胺凝胶电泳将蛋白质分离开来[1610固相pH梯度(immobilizedpH gr...
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采用等电聚焦电泳 Isoelectric Focus
用等电聚焦电泳法测得其等电点约为7.2。 结论:从栖热菌中提取的耐热乳糖酶的理化性质不同于其它来源的乳糖酶。
Its pI was about 7.2. Conclusion:The thermostable lactase from strain of thermus sp A3 was different from lactases from other microorganism.
用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35;
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
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