等电聚焦电泳(Isoelectric focusing (IEF), also known as electrofocusing)是一种电泳方法,即利用一种特殊的缓冲液(两性电解质)在凝胶(常用聚丙烯酰胺凝胶)内制造一个pH梯度,电泳时每种蛋白质就将迁移到等于其等电点(pI)的pH处(此时此蛋白质不再带有净的正或负电荷),形成一个很窄的区带。
环境水系分离嗜肺军团菌脉冲场凝胶电泳的分型研究-T... ... 橡树岭军团菌 - Legionella oakridgensis 等电聚焦电泳 - IFE,isoelectric focusing e 细菌素分型 - bacteriocin typing ...
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毛细管等电聚焦电泳 capillary isoelectric focusing ; CIEF
超薄层等电聚焦电泳 UTLIEF
等电聚焦电泳仪 Protein isoelectric system
滚动式等电聚焦电泳 RPIE
胺凝胶等电聚焦电泳 ULPAGIF
等电聚焦电泳法 Isoelectric Focusing-PAGE ; isoelectric focusing ; IEF-PAGE ; IEF
超薄等电聚焦电泳 UTLIEF
用等电聚焦电泳 isoelectric focusing ; IEF
超薄等电聚焦电泳技术 ultrathin-layer isoelectric focusing of proteins
In DE_(52) chromatography hyperplastic nodles bearing rat liver GST_S was separated to three isoenzymes named as C_(DE), A1, A2 in turn, PI of them measured by isoelectric focusing electrophoresis are 7.8, 6.7 and 6.3 respectively.
等电聚焦电泳测定等电点分别为7.8、6.7及6.3。
参考来源 - 大鼠正常肝、肝癌前病变组织谷胱甘肽SAll sample performed isoelectric focusing electrophoresis (IEF), separated by isoelectric point (pI); progressed sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and separated by protein molecular weight (Mr).
各试验组提取蛋白样本后行等电聚焦电泳(IEF),随后行十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),进行第二次分离。
参考来源 - CNV大鼠视网膜—脉络膜及缺氧人RPE细胞异常表达蛋白质的筛选与鉴定·2,447,543篇论文数据,部分数据来源于NoteExpress
采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。
Haemoglobin polymorphism of Chinese miniature horses was examined by agarose gel isoelectric focusing.
用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35;
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法,分析了栗属种的PGI同工酶的遗传。
Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method.
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