此外可运用该技术中缺口修复的方法构建质粒。
In addition, constructed plasmids can be by gap repair of this technology.
同时,斯蒂尔想通过增强自己号召力来修复党内资金缺口的算盘并不如意,RNC网站上对昔日著名黑人、拉丁美人及女性共和党人的赞美远远超过对他自己的赞美。
Meanwhile Mr Steele’s big idea, to restore the party’s fortunes by broadening its appeal, has not got much beyond a paean to famous black, Latino and female Republicans of yore on the RNC’s website.
在其他一些地区,很多人在躲避了早先的洪水之后聚集在那里。一处河堤决口,救援工人们正在竭尽全力的修复大堤上的这处缺口。
In another area, where people had gathered after escaping earlier floods, a canal had burst its Banks, and relief workers were struggling to repair the damage.
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