1974年，Rudolf Jaenisch通过将SV40 病毒的DNA注射到小鼠的囊胚中，创造了第一只携带外源基因的小鼠。后来又有研究人员把Murine leukemia病毒注射到小鼠胚胎得到了能通过生殖系统稳定遗传的小鼠，并且外源基因能在后代中稳定表达。这些能稳定遗传且表达外源基因的小鼠即我们一般意义上所说的转基因小鼠。
本发明还涉及在转基因小鼠中响应抗原激发产生全功能性仅有重链的抗体的方法。
The present invention also relates to a method of generation of fully functional heavy chain-only antibodies in transgenic mice in response to antigen challenge.
目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。
Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites.
结论：HNYCF可以改善APP转基因小鼠的学习记忆能力，其作用机制可能与抗炎、抗氧化作用有关。
CONCLUSION: HNYCF evidently ameliorates the learning and memory ability in APP transgenic mice, which may be related to the anti-inflammatory and anti-oxidation effects of HNYCF.
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