方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
Method:Two different methods, conventional CTAB method and modified CTAB method, were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定dna的损伤程度。
Methods: DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis.
经琼脂糖凝胶电泳分析载体与DNA结合力,并通过紫外分光光度计检测其载药量和包埋率;
The potential of adsorbing DNA on nanoparticles was analyzed by agarose gel electrophoresis and spectrophotometer.
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