Method:With Caenorhabditis elegans as the study material,we compared several nematode surface coat proteins extraction methods to determine their efficacy in separating nematode SCPs by SDS-PAGE and 2D-PAGE.
方法:以秀丽隐杆线虫(Caenorhabditis elegans)为研究材料,对比和分析不同的蛋白提取沉淀方法,进而采用SDS-PAGE电泳技术和双向电泳技术对所提蛋白进行评价。
参考来源 - 线虫surface coat proteins提取方法的建立与双向电泳分析·2,447,543篇论文数据,部分数据来源于NoteExpress
So in this paper we adopt 2D-PAGE to analysis the different serum protein group of healthy rat , cardiac muscle bloodless rat, treated drug-taken rat so that we may discuss the medication effect.
采用双向电泳技术分析了正常大鼠、心肌缺血模型大鼠、药物处理后的大鼠的血清蛋白组的差异,从而探讨利用双向电泳技术分析药物处理的有效性。
Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳(2 D PAGE)与质谱(MS)是分辨和鉴定蛋白的最常用的方法,但也存在有缺点。
Methods nuclear matrix proteins were extracted under high salt extraction procedures, and changes of nuclear matrix proteins were analysed by SDS PAGE and two dimensional (2d) electrophoresis.
方法应用高盐提取法抽提细胞核基质蛋白,SDS PAGE、双相电泳(2d)法分析白血病细胞与正常骨髓细胞核基质蛋白的变化。
应用推荐