Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.

方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。

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Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.

方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。

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The cultivation on microcarriers (MCs) is a perfect method on the large-scale animal cell culture, which is the complete combination of adhesive culture and suspending culture.

微载体培养法是一种将贴壁培养和悬浮培养完全融为一体的较为理想的动物细胞大规模培养方法。

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