...骨机械法分离培养成骨细胞,成骨细胞利用碱性磷酸酶染色(Alkaline phosphatase staining,ALP)、茜素红染色(Alizarin Red staining)进行鉴定。
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Oil red staining and alizarin red staining both demonstrated positive reactions but not in control groups.
成骨诱导后茜素红染色阳性，对照组为阴性。
The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .
运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
The osteogenic potential of the cell-CHA scaffold constructs cultured in RCCS were investigated by Alizarin Red S(ARS) staining, MTT method and histochemistry at 14th .
通过茜素红（ARS）染色，MTT法及其他组织化学的方法来研究在RCCS中培养14天时力学环境对成骨细胞功能状态的影响及其三维培养的研究的成骨潜能。
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