In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity, a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.
为提高碱性磷酸酶（ALP）活性测定的灵敏度，对以4-甲基伞形酮磷酸酯（4-MUP）为底物的荧光法进行了研究。
Alkaline phosphatase (ALP) activity was evaluated by thymolphthalein release from thymolphthalein monophosphate and matrix mineralization by staining with alizarin red S.
用磷酸麝香草酚酞中释放麝香草酚酞方法检测碱性磷酸酶ALP活性用硫酸茜红素染色法检测基质矿化。
Alkaline phosphatase (ALP) activity of osteoblasts was determined by enzyme dynamical method at 2d, 4d and 6d .
采用酶动力法测成骨细胞在基质材料上培养2，4，6 天后的碱性磷酸酶（ALP）活性。
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