Orthogonal design improved experimental efficiency and precision, which was a good way for selection and optimization of callus induction medium and differentiation medium.
正交设计可提高试验效率及准确性,是愈伤组织诱导培养基、分化培养基筛选优化的好方法;
With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.
以该配方诱导幼胚,处于胚胎选择阶段和组织与器官分化阶段的幼胚,能诱导出松软、晶莹透明的愈伤,诱导频率为2-6%。
It was important for callus induction and shoot differentiation to choose better starting materials and a suitable medium in rice anther culture.
选择合适的水稻花培起始材料,选用适宜的培养基配方,对于水稻花培中愈伤组织诱导和分化至关重要。
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