含有特异性前体细胞的样品, 首先用羟基荧光素二醋 酸盐琥珀酰亚胺酯(carboxyfluorescein diacetate succinimidyl ester , CFSE) 或 PKH26 (一种长链脂肪酸染料) 进行荧光染 色, CFSE可与胞内的氨基结合, PKH26...
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方法:用活细胞荧光染料羧基荧光素乙酰乙酸琥珀酰亚胺酯(carboxyfluorescein diacetate succinimidyl ester,CFDA-SE)标记小鼠B16-F10黑色素瘤细胞后,将肿瘤细胞分别用Nrp-1阻断抗体及Nrp-1配体血管内皮生长...
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而上述这些情况可以用羧基荧光素乙酰乙酸(carboxyfluorescein diacetate succinimidyl ester,CFSE)示踪和定量的流式细胞仪方法加以区分。
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Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
探讨应用活细胞荧光染料 CFSE 检测 T 淋巴细胞各亚群增殖反应的方法学。
Objective To investigate the feasibility of labeling iris pigment epithelial(IPE)cells of rabbits with 5-(and 6-)carboxyfluorescein diacetate succinimidyl ester(CFSE).
目的探讨活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯（CFSE）标记兔眼虹膜色素上皮（IPE）细胞的可行性。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
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