Subtractive cDNA library 消减cDNA文库
subtractive cDNA library construction 差减cDNA文库构建
forward subtractive cdna library 正向消减cdna文库
cDNA selected-subtractive suppression library cDNA差减抑制杂交文库
subtractive library of cdna 消减文库
suppression subtractive hybridization cDNA library 抑制差减杂交cDNA文库
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
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