The expression of VEGF in Tanshinone IIAtreated group was significantly lower than that in control group (P<0.01). The similar result could be observed in cell culture fluid with ELISA assay.
免疫细胞化学染色及ELISA法检测发现，丹参酮IIA作用组肝癌细胞VEGF表达和培养液中VEGF分泌量明显减少，与未加药对照组相比差异有统计学意义（P<0.01）。
The loose callus were choosed and transfered into fluid suspension culture, free cells and cell aggregate were obtained.
选择较松散的愈伤组织转入液体悬浮培养，获得游离细胞和细胞聚集体。
The culture fluid of the invention cultures cell with higher amplification times and longer generation time.
采用本发明的培养液培养细胞具有较高扩增倍数和较长传代时间。
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