The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .
运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
The transient expression of JEV E in BHK 21 cells was detected by indirect immunofluorescence assay(IFA) with a monoclonal antibody 2H4 against JEV E.
用其体外转染地鼠肾细胞BHK-21，经间接免疫荧光法（IFA）检测，证实了JEV-E的瞬时表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
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