Methods: The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification.

方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；

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In HEK293T, Dual-Luciferase Reporter Assay System was used to evaluate the effect of E4B on TLR pathway, and co-immunoprecipitation was used to identify interactive protein.

在HEK293T细胞中，通过双荧光报告系统检测E4B对TLR信号通路的影响，并且用免疫共沉淀的方法鉴定E4B的相互作用蛋白质。

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