The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
Experimental results further showed that dead volumes were also related to factors, such as the characteristics of the mobile phase, its composition and the column temperature.
结果表明死体积与流动相种类、配比和柱温等因素有关。
METHODS: Fixing other conditions than the composition and ratio of the mobile phase, estimate the optimum according to the separation results.
方法：固定其他色谱条件，改变流动相组成与配比，以分离结果的优劣优化流动相。
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